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. 1998 Nov 10;95(23):13709–13713. doi: 10.1073/pnas.95.23.13709

Table 1.

Genotype of embryos obtained from heterozygons mating

Age Number of genotype
+/+ or +/− −/−
Neonate 100 0
E16.5 30 0
E10.5 47 10
E9.5 30 6
E8.5 26 8
E7.5 20 7

The genotype was determined either by Southern blot analysis with E9.5–E16.5 embryos and neonates or by PCR analysis with E7.5 or E8.5 embryos.