Table 1.
Age | Number of genotype
|
|
---|---|---|
+/+ or +/− | −/− | |
Neonate | 100 | 0 |
E16.5 | 30 | 0 |
E10.5 | 47 | 10 |
E9.5 | 30 | 6 |
E8.5 | 26 | 8 |
E7.5 | 20 | 7 |
The genotype was determined either by Southern blot analysis with E9.5–E16.5 embryos and neonates or by PCR analysis with E7.5 or E8.5 embryos.