Figure 3.

eyg expression. Embryos were hybridized with a digoxigenin-labeled RNA probe generated from a full-length eyg cDNA. (a) Expression begins in early stage 11 in the SGP. (b–f) eyg expression appears in the head and torso slightly later and persists through germ-band retraction. (e, f, and j) During later stages of development, eyg expression remains in the head. (g–i) These ventral views of the embryo focus on the SGP. Expression begins in nonuniform patches (g) and soon forms a uniform patch outlining the SGP (h). It is transient and begins to fade during stage 12, leaving a crescent-shaped patch of expression before fading (i). Embryos double-stained with anti-En antibody (b and d, in brown) show how eyg is expressed in PS2. Expression in the torso alternates and does not overlap with the En stripes (f). During later stages of development, eyg is expressed in several regions of the head (j). These cells may be neurons or may become sensory organs in the larva.