Regulation of eyg expression in mutants. Embryos were hybridized with RNA probes for eyg and lacZ. Scr determines the identity of PS2 and the anteroposterior position of the SGP in the embryo. In an Scr mutant embryo, no salivary placodes are formed, and eyg expression in the SGP is lost, except for a small patch of cells that correspond to the PS1/PS2 border (a) Expression in other segments and in the head is unaffected. In a teashirt− mutant embryo, Scr is ectopically expressed in PS3, and eyg expression is also expanded in PS3 (b) In a dpp− mutant embryo, eyg expression expands dorsally to form an incomplete ring that is only interrupted ventrally (c) dpp− embryos fail to gastrulate properly and the embryos appear twisted. (d) In a sim− embryo, the SGPs on each side fuse, and eyg expression expands ventrally and crosses the midline to form a band.