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DNA Binding of Eyg RED and HD. (A) Gel shift with the optimal HD (P2CG) or RED-domain (5aCON) sites bound by the HD or RED domains of Eyg. (B) Precipitation of radioactively labeled oligonucleotides containing various RED-binding sites by an Eyg GST-RED-domain fusion protein attached to glutathione beads. (C) Alignment of the various binding sites containing subsets of the Pax-6 5aCON sites or the consensus obtained by the SELEX experiment.
