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Allele-specific PCR analysis for the detection of loss of the wild-type Aprt allele in Aprt mutant clones. Loss of heterozygosity in A, DMBA-induced 8-AAr clones; in B, ENU-induced 8-AAr clones; in C, x-ray-induced 8-AAr clones. All lanes represent different mutant clones. The normal allele is shown as a 300-bp fragment; the targeted allele is shown as a 170-bp fragment.
