Figure 3.

DSHP expression in normal reactive lymph node and malignant lymphoma. (a) Strong expression of DSHP in some germinal center (g) and paracortical (p) cells as demonstated by RNA in situ hybridization using antisense probe. Cells of mantle zone (m) do not express DSHP. (Inset) High magnification of strongly expressing cells with activated morphologic features of vesicular nucleus and nucleolus. Hybridization using sense probe was negative. Immunohistochemical analysis of this section demonstrates (b) the distribution of B cells using antibody to the marker CD30 and (c) the distribution of T cells using antibody to CD3. (d) Strong expression of DSHP in atypical cells of diffuse large B cell lymphoma.