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. 1997 Nov 11;94(23):12268–12273. doi: 10.1073/pnas.94.23.12268

Figure 1.

Figure 1

Results of protein-DNA photocrosslinking within the TBP-IIB-IIF-RNAPII-promoter complex. (A) Representative data from standard photocrosslinking experiments (positions −24 to −8 of DNA template strand). (B) Representative data from control “in-gel” photocrosslinking experiments (positions −24 to −8 of DNA template strand). (C) Representative data from control experiments by using IIF-RAP30* and IIF-RAP74* (positions −18 and −14 of DNA template strand; see Materials and Methods). (D) Summary of results (results for nontemplate strand above sequence; results for template strand beneath sequence). Phosphates analyzed are indicated by ∗. Sites exhibiting reproducible crosslinking are indicated by solid bars; sites exhibiting less reproducible crosslinking are indicated by shaded bars (scoring based on data from 2–5 independent standard photocrosslinking experiments). The TATA element and transcription start are indicated by shading.