Skip to main content
. 1998 Nov 10;95(23):13806–13811. doi: 10.1073/pnas.95.23.13806

Figure 2.

Figure 2

(A) Pre-exposure to TNF inhibits IFN-γ priming for enhanced IL-12 production. Elicited macrophages were incubated with cytokines simultaneously with (Co-Rx, Upper), or 16 hr before (Pre-Rx, Lower), the addition of LMW-HA. Conditioned media was collected after 32 hr. ∗, P < 0.05 compared with IFN-γ-primed, HA-stimulated cultures. (B) Inhibition of IFN-γ priming is blocked by neutralizing anti-TNF antibody. Elicited macrophages were treated with cytokines in the presence of anti-TNF antibody (10 μg/ml) or control antibody (53.672; 10 μg/ml) for 16 hr before the addition of LMW-HA for an additional 16 hr. (C) Role of TNFR p55 and TNFR p75 in TNF-mediated inhibition of IFN-γ priming. Elicited macrophages were pretreated with antibodies specific for the p55 or the p75 subunit of the TNFR (10 μg/ml) or isotype-matched control antibody (2C11, 10 μg/ml) for 45 min at 4°C. Cells were washed and stimulants added. (D) Elicited macrophages were harvested from TNF−/− mice. Cells were treated with the indicated cytokines 16 hr before the addition of LMW-HA. ∗, P < 0.05 compared with IFN-γ-primed cultures. IL-12 p70 levels were measured by RIA.