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. 1998 Nov 10;95(23):13806–13811. doi: 10.1073/pnas.95.23.13806

Figure 3.

Figure 3

(A) TNF-mediated inhibition of IFN-γ-primed IL-12 production is dose dependent. Cells were treated with indicated doses of TNF and 500 units/ml IFN-γ 16 hr before addition of LMW-HA. IL-12 p70 production was measured by RIA. (B) Pre-exposure to TNF inhibits IFN-γ priming for enhanced IL-12 production to LPS and HA. Elicited macrophages were incubated with the indicated cytokines 16 hr before stimulation with LMW-HA (100 μg/ml) or LPS (1 μg/ml) for an additional 16 hr. IL-12 p70 levels were measured by RIA. ∗, P < 0.05 compared with IFN-γ-primed cultures. (C) TNF-mediated inhibition of IFN-γ-primed, HA-induced IL-12 p40 mRNA accumulation. Elicited macrophages were incubated with the indicated cytokines for 16 hr before the addition of LMW-HA for 8 hr. Total RNA was extracted and the levels of IL-12 p40 mRNA were detected by Northern blot. Aldolase controls are shown. ∗, P < 0.05 compared with lane 4.