Table 2.
Medicago reference genes and primers for qRT-PCR
| Gene Name | TC |
Accession Number |
Forward/Reverse Primer (5'-3') |
PCR Product Size (bp) |
PCR efficiency (E) |
R2 |
| β Tubulin | TC106341 | N | TTTGCTCCTCTTACATCCCGTG / GCAGCACACATCATGTTTTTGG | 100 | 1.08 | 1.00 |
| PPRrep | TC96273 | N | GGAAAACTGGAGGATGCACGTA / ACAAGCCCTCGACACAAAACC | 100 | 0.93 | 1.00 |
| PDF 2 | TC107161 | N | GTGTTTTGCTTCCGCCGTT / CCAAATCTTGCTCCCTCATCTG | 100 | 0.99 | 1.00 |
| bHLH | CX538576 | N | TAGCGAGTACCATGATGCCAGA / GCGCCTCTTTTGTTTTCAGC | 100 | 0.89 | 1.00 |
| UBC | AW686873 | N | CTGACAGCCCACTGAATTGTGA / TTTTGGCATTGCTGCAAGC | 100 | 0.96 | 1.00 |
| PTB | TC111751 | N | CGCCTTGTCAGCATTGATGTC / TGAACCAGTGCCTGGAATCCT | 100 | 0.85 | 1.00 |
| Ubiquitin | TC102473 | AC137828_19.4 | GCAGATAGACACGCTGGGA / AACTCTTGGGCAGGCAATAA | 100 | 0.95 | 1.00 |
| UBC9 | TC106312 | AC137602_2.4 | GGTTGATTGCTCTTCTCTCCCC / AAGTGATTGCTCGTCCAACCC | 100 | 1.13 | 0.99 |
| Helicase | CB892427 | N | GTACGAGGTCGGTGCTCTTGAA / GCAACCGAAAATTGCACCATAC | 100 | 0.91 | 1.00 |
| ELF1α | EST317575 | N | GACAAGCGTGTGATCGAGAGATT / TTTCACGCTCAGCCTTAAGCT | 100 | 0.68 | 0.98 |
| UPL7 | TC111218 | N | CCAGTTGTTCTCGTGGTCCATT / CCTCCAATTGTCGCCCAAA | 100 | 0.93 | 1.00 |
| GAPDH | MtC00030_GC | CT573421_3.4 | TGCCTACCGTCGATGTTTCAGT / TTGCCCTCTGATTCCTCCTTG | 100 | 1.04 | 0.99 |
| Actin2 | TC107326 | AC137836_27.5 | TCAATGTGCCTGCCATGTATGT / ACTCACACCGTCACCAGAATCC | 100 | 1.12 | 0.99 |
| MSC27 | X63872 (M. sativa) | N | GTTGAAGTAGACATTGGTGCTAACG / AGCTGAGTCATCAACACCCTCAT | 100 | 0.76 | 0.99 |
Mean PCR efficiency (E) was determined from three biological replicates of each of six organs, using LinRegPCR [31], which also yielded mean R2. N, no corresponding GenBank accession number.