Figure 3.

Effect of NSC-87877 on agrin-induced dispersal of pre-patterned AChR clusters. Muscle cells labeled with R-BTX were incubated overnight in culture medium containing agrin (ctl; A, B), agrin plus 2 μM NSC-87877 (NSC; C, D), or agrin plus 20 μM pervanadate (PV; E, F). In agrin-treated muscle cells AChR micro-clusters (~0.5–2 mm in diameter) were found but larger clusters were not generally detected (B). However, in cells incubated with agrin and NSC (D) or agrin and PV (F), both micro-clusters and large pre-patterned clusters were present (arrows).