Table 1.
Primers used for the detection of HPV DNA in cervical cancer tissues
| Name | Target DNA | Nucleotide sequence(5'-3') | Prime position | Product size(bp) | |
| Primers for the One-step TS PCR and the second round of the Nested TS PCR* | |||||
| 1601 | Sense | HPV16 E6 to E7 gene | CACAGTTATGCACAGAGCTGC | 141–161 | 457 |
| 1602 | Antisense | CATATATTCATGCAATGTAGGTGTA | 597–573 | ||
| 1801 | Sense | HPV18 E6 gene | CACTTCACTGCAAGACATAGA | 170–-190 | 322 |
| 1802 | Antisense | GTTGTGAAATCGTCGTTTTTCA | 491–470 | ||
| 5201 | Sense | HPV52 E6 gene | TAAGGCTGCAGTGTGTGCAG | 178–197 | 229 |
| 5202 | Antisense | CTAATAGTTATTTCACTTAATGGT | 406–383 | ||
| 5801 | Sense | HPV58 E6 gene | GTAAAGTGTGCTTACGATTGC | 297–317 | 274 |
| 5802 | Antisense | GTTGTTACAGGTTACACTTGT | 570–550 | ||
| 5901 | Sense | HPV59 E6 gene | CAAAGGGGAACTGCAAGAAAG | 159–179 | 215 |
| 5902 | Antisense | TATAACAGCGTATCAGCAGC | 373–354 | ||
| Primers for the first round amplifications of Nested TS PCR** | |||||
| LF16 | Sense | HPV 16 URR to E7 gene | AGGGCGTAACCGAAATCGGT | 27–45 | 632 |
| LR16 | Antisense | CTGAGCTGTCATTTAATTGCTCA | 658–636 | ||
| LF18 | Sense | HPV 18 URR to E7 gene | GGGAGTAACCGAAAACGGT | 35–53 | 662 |
| LR18 | Antisense | TCCTCTGAGTCGCTTAATTGCTC | 696–674 | ||
| LF52 | Sense | HPV 52 URR to E7 gene | GGGTGTAACCGAAAACGGT | 31–49 | 619 |
| LR52 | Antisense | CTGAGCTGTCACCTAATTGCTCA | 649–627 | ||
| LF58 | Sense | HPV58 URR to E7 gene | GGGTGTAACCGAAAACGGT | 33–51 | 638 |
| LR58 | Antisense | CTGAGCTGTCACATAATTGCTCA | 670–648 | ||
| LF59 | Sense | HPV59 URR to E7 gene | GTTAAGACCGAAAACGGTG | 1–19 | 648 |
| LR59 | Antisense | GAGTCGGAGTCAGGTAATTGCTC | 648–626 | ||
* designed by Sotlar et al.[17]; **Designed specifically for this study.