Figure 5.

XC2976 is required for Xcc zinc resistance and the XC2976 and zur mutants display a similar zinc sensitivity level. Two hundred microliters of overnight culture (cell concentration adjusted to OD₆₀₀ = 1.0) of each Xcc strain were inoculated into 200-ml NYG medium supplemented with ZnSO₄ to a final concentration of 300, 400 or 500 µM. Cells were incubated at 28°C with shaking at 200 r.p.m. for 24 h. The cell density was measured spectrometrically at 600 nm. Values are the mean ± SD of triplicate measurements. Each experiment was repeated three times and similar results were obtained.