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. 2008 Jul 2;36(13):4424–4432. doi: 10.1093/nar/gkn401

Figure 3.

Figure 3.

Comparison of the antisense activity of bis-phosphonate-conjugated PNAs, phosphonate-conjugated PNAs and PNA/DNA heteroduplex delivered by lipofection. Three PNAs conjugated to 4, 5 and 6 units of bis-phosphonate monomers, respectively (bP4-PNA, bP5-PNA and bP6-PNA) and two phosphonate-conjugated PNAs (P7-PNA, and P10-PNA) and PNA/DNA heteroduplex were complexed with LipofectAMINE2000 and used for delivery to the HeLa pLuc705 cells. After 24 h, the cells were subjected to the further analysis. (A) Luciferase activity [using Bright-Gro assay (Promega)]. The values represent the mean ± SD of three experiments. (B) RT-PCR analysis of the mis-splicing correction of luciferase pre-mRNAs by phosphonate-conjugated PNAs. U, fragments without correction (268 bp). (C) Fragments with mis-splicing correction (142 bp). The numbers under the figure indicate the relative amount (%) of the corrected form to the sum of corrected form and uncorrected form.