Table 1.

PNA oligomers

No.	Name	Sequence	Mass found (calcd)	(%)a
2389	Naked-PNA	H-CCTCTTACCTCAGTTACA-NH2	4767 (4765)	95+
3283	P3-PNA	H-(Gln-phosphonateb)3-eg1-CCTCTTACCTCAGTTACA-NH2	5576 (5576)	90+
3242	P7-PNA	H-(Gln-phosphonate)7-eg1-CCTCTTACCTCAGTTACA-NH2	6482f (6464)	80+
3284	P10-PNA	H-(Gln-phosphonate)10-eg1-CCTCTTACCTCAGTTACA-NH2	7127f (7128)	#
3323	bP4-PNA	H-(Lys-bis-phosphonatec)4-CCTCTTACCTCAGTTACA-NH2	6181f (6196)	95+
3324	bP5-PNA	H-(Lys-bis-phosphonate)5-CCTCTTACCTCAGTTACA-NH2	6545f (6554)	95+
3325	bP6-PNA	H-(Lys-bis-phosphonate)6-CCTCTTACCTCAGTTACA-NH2	6915f (6912)	90+
3326	Flk-bP6-PNAd	H-Flk-(Lys-bis-phosphonate)6-CCTCTTACCTCAGTTACA-NH2	7427f (7441)	95+
2745	PNA-mismatche	H-eg1-CCTCTGACCTCATTTACA-NH2	4911 (4910)	90+
3353	bP6-PNA-mismatche	H-(Lys-bis-phosphonate)6-CCTCTGACCTCATTTACA-NH2	6909 (6912)	95+

^aPurity (%): 95+ corresponds to single peak with slight foot; 90+ corresponds to single peak with slight foot and/or shoulder peak; 80+ corresponds to single peak with significant foot and/or shoulder peaks; #Broad peak with shoulders, exact purity difficult to estimate.

^bStructure is shown in Figure 1.

^cStructure is shown in Figure 1.

^dFl-bP6-PNA has fluorescein (Fl) attached to the ε-amino group of a lysine residue (39).

^eMismatch PNAs of PNA2745 and PNA3353, whereas two mismatches are underlined.

^fDue to the polyanionic character pf the phosphonate-conjugated PNAs, the MALDI signal from these were quite weak and broad, and therefore less accurate than for the unmodified PNAs.