Table 2.

Thermal stability (T_m) of PNA–DNA and –RNA duplexes

Antisense Oligo#	Name	Tm (°C)a
		Complex with sense DNAb	Complex with sense RNAc
PNA2389	Naked PNA	69.0	79.0
PNA 3283	P3-PNA	70.1	78.1
PNA3242	P7-PNA	68.0	77.2
PNA3284	P10-PNA	68.2	78.1
PNA3323	bP4-PNA	69.0	79.0
PNA3324	bP5-PNA	69.1	78.3
PNA3325	bP6-PNA	68.1	78.0
PNA2745	PNA-mismatch	45.0	59.0
PNA3353	bP6-PNA-mismatch	46.1	59.2
DNA2389d	DNA2389	58.0	60.0
2′O-MeRNAe	2OMe	56.0	75.0

^aMelting temperatures of duplexes were measured in 10 mM sodium phosphate (pH 7.0) containing 100 mM NaCl and 0.1 mM EDTA.

^bSense DNA sequence, 5′-TGTAACTGAGGTAAGAGG-3′.

^cSense RNA sequence, 5′-UGUAACUGAGGUAAGAGG-3′.

^dDNA2389 sequence, 5′-CCTCTTACCTCAGTTACA-3′.

^e2′O-MeRNA sequence, 5′-CCUCUUACCUCAGUUACA-3′.