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. 2008 Jun 27;36(13):4317–4326. doi: 10.1093/nar/gkn383

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The initial rate of GFP₂-IN NLS import is reduced in classical nuclear protein import mutants. The initial rate of nuclear import of GFP₂-IN NLS is dramatically reduced in gsp1-1, rna1-1, prp20-1, srp1-55 and rsl1 L63A cells relative to wildtype cells. Wildtype, gsp1-1, rna1-1, prp20-1, srp1-55 and rsll L63A cells containing GFP₂-IN NLS were analyzed using a semi-quantitative kinetic import assay as described in the ‘Materials and Methods’ section (43). Import in wildtype cells was analyzed at both 37 and 18°C. Data obtained was identical at both temperatures and results are shown only for the 37°C sample. The percentage of cells with GFP₂-IN NLS nuclear accumulation was plotted versus time to obtain an approximation of initial import rates.