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. 2008 Jun 17;36(13):e79. doi: 10.1093/nar/gkn377

Figure 2.

Figure 2.

pWGA reaction time, product length, and product yield. (A) pWGA reactions containing 10 ng human genomic DNA were terminated after a specified incubation time [0 (lanes 2–4), 30 (lanes 5–7), 60 (lanes 8–10), 90 (lanes 11–13) or 120 (lanes 14–16) minutes], as indicated at the top of the gel, and 5 µl of each 25 µl reaction was analyzed on a 0.6% agarose gel. The reactions were performed in triplicate, and lane 1 shows 500 ng of 1-kb DNA ladder (NEB) with the sizes of bands indicated on the left of the gel. The lane numbers are indicated at the bottom of the gel. (B) Quantification of pWGA product yield by PicoGreen® assay. Fluorescent intensity of 0.1, 0.5, 1, 5 and 10 ng of un-amplified DNA (magenta diamonds) is plotted against the amount of DNA and is fitted to the linear equation shown at the bottom right of the plot (black line) as a standard curve for quantification. Triplets of pWGA product from 1 ng (orange triangle) and 10 ng (blue circle) were diluted 20 times. One microliter of the diluted products were assayed for PicoGreen® fluorescence and mapped on the standard curve according to their intensity. The ± SD over three experiments are shown as error bars on each data point.