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Representative sequencing results using the pWGA amplification product as template. The template for sequencing was the pWGA amplification product from plasmid pET28a(+). The primer used for sequencing was the T7 terminator primer (S1271, NEB) that covers residues 66–84 on the plasmid and it is in the reverse direction of the plasmid. The sequencing experiment was performed by Dye-Terminator Sequencing method using AmpliTaq® DNA polymerase FS on an ABI 3130xl Genetic analyzer.
