Table 1.

Using pWGA product as template for DNA sequencing

Plasmid		Sequencing primer				Continuous accurate sequence read
Name	Length	Direction	Name	Start	Primer sequence (5′–3′)	Start–End	Length
pTYB3	7477	Forward	S1248	5637	TAATACGACTCACTATAGGG	5691–6317	627
		Reverse	S1261	5862	ACCCATGACCTTATTACCAACCTC	5824–5100	725
pET28a(+)	5369	Forward	S1271	66	TATGCTAGTTATTGCTCAG	135–799	665
		Reverse	S1248	386	TAATACGACTCACTATAGGG	331–1, 5369–4968	733
pET15b	5708	Forward	S1271	253	TATGCTAGTTATTGCTCAG	280–897	618
		Reverse	S1248	479	TAATACGACTCACTATAGGG	424–1, 5708–5490	643