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Limited proteolysis analysis of wild-type TetR and revTetR variants with subtilisin. Whereas in wild-type TetR, the presence or absence of the effector does not alter the proteolytic susceptibility of TetR, the revTetR variants are only protected against proteolysis in the presence of the effector ATC or [Mg-ATC]+. The four asterisks mark protein bands that were N-terminally sequenced.
