Figure 5.

(A) HDAC1 knockdown in HepG2 cells by specific siRNA. HepG2 cells were transfected with indicated amounts of control and HDAC1 siRNA (Smart Pool from Dharmacon Inc.). WCEs were isolated 72 h after transfection and Western immunoblots were performed with anti-HDAC1 Ab (upper panel). Lower panel shows expression of β Actin as internal control. (B) HDAC1 knockdown accumulates STAT3 in the nucleus. HepG2 cells were transfected with either control siRNA or HDAC1 siRNA for 72 h. IL-6 stimulated (20 min) NEs were prepared and separated by SDS–PAGE followed by Western immunoblot analysis with anti-STAT3 Ab, anti-AcLys87 STAT3 Ab, or anti-phospho-Tyr⁷⁰⁵ Ab. Lower panel shows expression of HDAC1 after HDAC1 siRNA knockdown. Band intensity were plotted and shown in right-hand side panel.