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Accumulation of nuclear STAT3 after HDAC1 knockdown. HepG2 cells were transfected with either control siRNA or HDAC1 siRNA. Seventy-two hours later cells were stimulated with IL-6 for indicated time. Cells were fixed and stained with either fluorescein isothiocyanate (FITC)-anti-STAT3 Ab. Staining with FITC-anti-AcK87 STAT3 Ab (data not shown) had similar result. Nuclei were stained with DAPI. Confocal immunofluorescence of representative cells is shown.
