Figure 7.

(A) HDAC1 knockdown activates IL-6 stimulated hAGT promoter activity in HepG2 cells. HepG2 cells were transfected with either control siRNA or HDAC1 siRNA along with (hAPRE1)5-LUC reporter. Forty-eight hours after transfection cells were stimulated with IL-6 for 24 h and then harvested for luciferase reporter assay. *P < 0.05 relative to control siRNA, t-test. (B) Silencing of HDAC1 activates IL-6 regulated MCP1 expression in human monocytic THP1 cells. THP1 cells were transfected with either control siRNA or HDAC1 siRNA. Thirty-six hours after transfection, cells were stimulated with IL-6 for indicated time and total RNA was extracted at the same time for real-time RT–PCR analysis. *P < 0.05 relative to control siRNA, t-test.