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. 2008 Jun 27;36(13):4352–4363. doi: 10.1093/nar/gkn364

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Destabilization of TAR results in increased in vitro dimerization of leader RNA. (A) In vitro transcribed leader RNA molecules (corresponding to nucleotides +1 to +368 in wild-type HIV-1) with either a wild-type (TAR^wt), mutant (TAR^m) or deleted TAR sequence (mutants A–F) were analyzed on a denaturing polyacrylamide gel. (B) The transcripts were allowed to dimerize in the presence of Mg²⁺. Monomeric (m) and dimeric RNAs (d) were analyzed on a native polyacrylamide gel containing Mg²⁺. (C) The monomeric and dimeric bands were quantified and dimerization efficiency was determined by calculating the fraction of RNA present as dimer. The average of 2–5 experiments is shown with arrow bars indicating the SD.