Figure 4.

Restriction endonuclease analysis of the BLAΔ¹⁶⁴⁴ library. (a) Rationale for determining MuDel (teal) insertion diversity within the bla gene (orange with black stripe) of pNOM. Digestion by XhoI (recognition site shown as blue triangle) of the pooled pNOM plasmid with MuDel inserted within the bla gene generates one major product of 3422 bp in size. Digestion with MlyI (recognition site shown as red triangle) generates two major products of 1310 bp (MuDel plus 8 bp from bla) and 2112 bp in size. Digestion with both XhoI and MlyI generates the 1310 bp MuDel fragment together with many different size fragments depending on the insertion position of the transposon. (b) Restriction analysis of the BLAΔ¹⁶⁴⁴ library with XhoI and/or MlyI, as indicated in the figure.