Table 2.

Examples of TEM-1 variants with enhanced properties in vivo^a

Amino acid substitutionb	Nucleotide changes	MIC fold changec
		CAZ	CLV
M69I	atg→atc	−	3
P145R	ccg→cga	4	−
D163E-R164N	gatcgt→gagaat	16	−
D163D R164S	gatcgt→gactct	32	−
D163D R164H	gatcgt→gaccat	64	−
D163D R164D	gatcgt→gacgat	32	−
R164P-W165R	cgttgg→cctagg	32	−
	cgttgg→ccgcgg	4	−
R164P-W165G	cgttgg→ccaggg	8	−
W165G	tgg→ggg	2	−
W165P	tgg→cct	−	2
W165Y	tgg→tac	−	3
	tgg→tat	−	3
E166G	gaa→gga	8	−
P167H	ccg→cac	8	−
E171P	gaa→cca	8	−
R178A	cgt→gca	4	−
E197P	gaa→cca	2	1.5
G238G E240R	ggtgag→ggcagg	4	−
S243S R244T	tctcgc→tccacc	−	3
R275V	cga→gta	−	8
	cga→gtg	−	8
	cga→gtt	−	8
R275T	cga→acc	−	2

^aThe full list of sequenced TEM-1 variants are shown in Supplementary Table 1.

^bNumbering according to standard system (25).

^cFold change compared to MIC observed for cells producing wt TEM-1 encoded in the pNOM plasmid (CAZ MIC 0.3 μg/ml, CLV MIC 4 μg/ml).