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. 2008 Jul 4;36(13):4474–4487. doi: 10.1093/nar/gkn411

Table 1.

Primers used in the generation of PCR products that served as templates in in vitro transcription. Numbering starts from the beginning of 3′-UTR

AT1R 3′-UTR sense primers (number denotes the first nucleotide of the 3′-UTR included in the oligo)
1 TAATACGACTCACTATAGGG catgttc gaaacctgtc cataaagtaa ttttgtgaaa gaagg.
100 TAATACGACTCACTATAGGG gaattga aggagaaaat gcattatgtgg
118 TAATACGACTCACTATAGGG cattatgtg gactgaaccg acttttctaa
121 TAATACGACTCACTATAGGG tatgtg gactgaaccg acttttctaa
150 TAATACGACTCACTATAGGG tctgaac aaaagctttt ctttccttttgcaacaagac
300 TAATACGACTCACTATAGGG agcctgc ttttgtcctg ttatttttta tttccacata aagg
600 TAATACGACTCACTATAGGG gtataat ggtgttacta aagtcacata taaaagttaa ac
AT1R 3′-UTR antisense primers (number denotes the last nucleotide included in the oligo)
100 ctgaaaagta gctaatgctc atttggtagt gaag
200 tgtggctttgctttgtcttgttgc
250 attcatcgagtttctgacattgttcttcgagcagcc
300 cagtaaaatt tctcaaatca acacattcat cgagtttc
600 attgttttgg cagtgtaaac ctataagaca caggttg
857 aaacaggaag gcatacttta tgatatataa tctttttaccac
Control (part of the luciferase coding region)
S TAATACGACTCACTATAGGG gcgccattctatccgctggaagatggaacc
AS cgcccaacaccggcataaagaattgaagag