Skip to main content
. 2008 Aug 1;283(31):21703–21713. doi: 10.1074/jbc.M800809200

FIGURE 7.

FIGURE 7.

The proteasome inhibitor MG-132 prevented spinal cord EAAC1 down-regulation. Rats (n = 6) were administrated intrathecally (twice daily) with vehicle, morphine (MS, 15 nmol), morphine plus MG-132 (5 nmol), or MG132 (5 nmol) alone for seven consecutive days. A, Western blotting of glutamate transporter EAAC1, PTEN, and Need4 within the spinal cord dorsal horn. B, the mRNA level of EAAC1, PTEN, and Nedd4 examined using RT-PCR after each treatment. C, densitometric quantification of RT-PCR results are shown in B. Analysis of variance, *, p < 0.05, as compared with the vehicle control. Lane 1, vehicle; lane 2, morphine (15 nmol); lane 3, morphine (15 nmol) plus MG-132 (5 nmol); lane 4, MG-132 (5 nmol) alone. Results are representative of three independent experiments or the mean ± S.E. from at least three independent extract preparations. Tubulin, loading control.