Skip to main content
. 2008 Aug 1;283(31):21462–21468. doi: 10.1074/jbc.M803150200

FIGURE 4.

FIGURE 4.

HFE expression promotes Zip14 degradation in HepG2 cells. A, HFE promotes Zip14 degradation in HepG2 cells. HepG2 cells and HepG2/HFE cells were treated with 100 μg/ml cycloheximide (CHX) for 0, 4, 8, and 12 h. Cell lysates were analyzed by immunoblot for Zip14 normalized to internal actin control using fluorescent secondary antibodies. The bands detected at ∼55 and ∼42 kDa represented Zip14 and actin, respectively. These results are representative of one of four experiments without significant variation between experiments. Endogenous Zip14 was degraded over time, and Zip14 degradation was promoted by HFE expression. B, quantitation of Zip14 degradation in HepG2 cells and HepG2/HFE cells. The amount of Zip14 was expressed as the ratio of the fluorescence intensity of Zip14 to actin. Zip14 expression in HepG2 cells and HepG2/HFE cells treated with cycloheximide for 0 h was set as 100%. Half-life was determined by linear regression analysis. Data are shown as average ± S.D. t½ was decreased in HFE HepG2 cells. Con, control.