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Egf1.0 and Egf10ΔCD dose-dependently inhibit processing of pro-PAP1 and pro-PAP3. Increasing amounts (0–200 pmol) of Egf1.0 (A–C) or Egf1.0ΔCD (D–F) were added to plasma followed 10 min later by the addition of curdlan. Samples were then subjected to immunoblot analysis using PAP1 (A and D), PAP3 (B and E), or SPN3 (C and F) antibodies. Sizes of the molecular mass markers are indicated to the left of each blot.
