FIGURE 6.

R1 and R5 are responsive to CsgA seeding. A–C, indicated percentages of sonicated CsgA fibers were added to freshly prepared 17 μm ΔR1 (A), 50 μm ΔR5 (B), and 50 μm ΔR1&5 (C). The polymerization was measured by ThT fluorescence. D, 2% sonicated CsgA fibers were added to freshly prepared 1.0 mg/ml R1 peptide solution. The polymerization was measured by ThT fluorescence. E, R5 was solubilized in HFIP/trifluoroacetic acid. Upon the removal of HFIP/trifluoroacetic acid, R5 was resuspended in 1.0 m GdnHCl buffered by 50 mm potassium phosphate buffer at pH 7.2. 4 and 8% sonicated CsgA fibers were added to 0.2 mg/ml R5 solution in the presence of 1.0 m GdnHCl and mixed with ThT at 20 μm, and fluorescence was measured. F, the indicated percentages of sonicated CsgA fibers were added to freshly prepared 1.0 mg/ml R3 peptide solution. The polymerization was measured by ThT fluorescence.