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. 2008 Aug 1;283(31):21540–21549. doi: 10.1074/jbc.M800214200

FIGURE 3.

FIGURE 3.

BH3 domain deletion construct of apoL1 failed to induce cell death in DLD-1 cells. A, adenoviruses harboring WT apoL1 (AD-apoL1) and BH3 domain deletion mutant of apoL1 (AD-apoL1.dBH3) were constructed and used to infect DLD-1 cells. Cells overexpressing green fluorescence protein also overexpressed apoL1 protein. AD-apoL1 induced cell death 72 h after infection (panels b (bright field) and e). In contrast, AD only or AD-apoL1.dBH3 failed to induce cell death (panels a (bright field) and d, and c (bright field) and f, respectively). B, time-dependent protein production of WT apoL1 and apoL1.dBH3. Hours after infection were as indicated. Note that endogenous expression of apoL1 was very low, almost undetectable, in DLD-1 cells (1st, 2nd, and 5th lanes). Both proteins were stably produced in time-dependent manner (3rd and 6th and 4th and 7th lanes for WT apoL1 and apoL1.dBH3, respectively). β-Actin was used as a loading control.