BH3 domain deletion construct of apoL1 failed to induce cell death in
DLD-1 cells. A, adenoviruses harboring WT apoL1 (AD-apoL1) and
BH3 domain deletion mutant of apoL1 (AD-apoL1.dBH3) were constructed and used
to infect DLD-1 cells. Cells overexpressing green fluorescence protein also
overexpressed apoL1 protein. AD-apoL1 induced cell death 72 h after infection
(panels b (bright field) and e). In contrast, AD only or
AD-apoL1.dBH3 failed to induce cell death (panels a (bright field)
and d, and c (bright field) and f, respectively).
B, time-dependent protein production of WT apoL1 and apoL1.dBH3.
Hours after infection were as indicated. Note that endogenous expression of
apoL1 was very low, almost undetectable, in DLD-1 cells (1st, 2nd,
and 5th lanes). Both proteins were stably produced in time-dependent
manner (3rd and 6th and 4th and 7th lanes
for WT apoL1 and apoL1.dBH3, respectively). β-Actin was used as a loading
control.