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. 2008 Aug 1;283(31):21540–21549. doi: 10.1074/jbc.M800214200

FIGURE 4.

FIGURE 4.

ApoL1-induced cell death is through autophagy, not by apoptosis. A, caspases 9 and 3 were not activated, and PARP was not cleaved during apoL1-induced cell death. By contrast, apoL6 induces cell death through apoptosis by activation of caspases 9 as described previously (45). B, apoL1 induced activation andtranslocationofLC3.ImmunoblotanalysisofLC3inautophagicDLD-1.apoL1 cells. Total soluble proteins were isolated at indicated time points from DLD-1.apoL1 cells cultured in induction medium. Times after induction of apoL1 are as indicated. Accumulation of LC3-II in induced DLD-1.apoL1 cells can be seen 12 h after induction. The ratios between LC3-II and LC3-I were 0.1, 2, and 20, 0, 12, and 24 h after induction, respectively. C, immunofluorescence staining of LC3 (red) in DLD-1.apoL1 cells. Panel a, DLD-1.apoL1 cells cultured in normal medium, and panel b, DLD-1.apoL1 cells cultured in induction medium for 24 h. At least 300 cells were analyzed for each experiment.