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Degradation of internalized 125I-HA by cells expressing 190-HARE mutants. Confluent cells expressing the indicated WT or HARE mutants were incubated at 37 °C with 1.5 μg/ml 125I-HA with or without 150 μg/ml unlabeled HA for 8 h. Medium samples were taken to assess released 125I-HA degradation products and normalized as described under “Experimental Procedures.” The values plotted are the mean ± S.E. (n = 6) percent degradation of the specifically internalized 125I-HA.
