TABLE 1.

Primer sequences used to create mutations in the 190-HARE CD Deletion or substitution mutations were made as described under “Experimental Procedures” using the indicated forward (F) and reverse (R) primers. Deletion of multiple motifs was accomplished by using a single, double, or triple motif deletion mutant HARE cDNA and a set of primers to delete codons specifying another motif.

190-HARE mutant	Forward and reverse mutagenic primer sequences
(ΔYSYFRI) = ΔM1	F, 5′-GTTGCCTTGGCTGCTAACCGGAGAAC-3′
	R, 5′-GATTGTTCTCCGGTTAGCAGCCAAGG-3′
(ΔFQHF) = ΔM2	F, 5′-CGGAGAACAATCGGCGAGTCGGAAGAGGAC-3′
	R, 5′-GTCCTCTTCCGACTCGCCGATTGTTCTCCG-3′
(ΔNPLY) = ΔM3	F, 5′-CAGCAGCCTGAGAATATCTCGGAGAGCACAACCTCAGCTCCC-3′
	R, 5′-GGGAGCTGAGGTTGTGCTCTCCGAGATATTCTCAGGCTGCTG-3′
(ΔDPF) = ΔM4	F, 5′-CCAGAACCTTCCTACACGGACTCTGAAGAAC-3′
	R, 5′-GTTCTTCAGAGTCCGTGTAGGAAGGTTGCTGG-3′
Y2519A	F, 5′-GAGAATATCTCGAACTTGGCTGAGAGCACAACC-3′
	R, 5′-GGTTGTGCTCTCAGCCAAGTTCGAGATATTCTC-3′