FIGURE 7.

Mislocalization of MVB cargo upon loss of Doa1. A, localization of GFP-Ub in vps27Δ cells and vps27Δ doa1Δ double mutant cells. Cells were counterlabeled with FM4–64. Shown also are the merged fluorescence images and DIC image. B, localization of GFP-Ub (pPL3601) in vps27Δ cells and vps27Δ doa1Δ double mutant cells. Cells were also expressing mCherry-tagged Ste3 (pPL3607). Shown also are the merged fluorescence images and DIC image. C, localization of Vph1-GFP-Ub (pPL1556) in wild type (WT) and doa1Δ mutant cells.