TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference/Source |
|---|---|---|
| pGO45 | pRS426 carrying GFP-CPS1 | Ref. 71 |
| pPL1867 | LEU2 conversion of pGO45 | This study |
| pPL3267 | HA-Ub-GFP-CPS1 in pRS316 | Ref. 20 |
| pPL3453 | HA-Ub-GFP-CPS1 in pRS315 | This study |
| pPL1124 | pRS316 containing dominant negative VPS4 | Ref. 20 |
| pGEX-6P-1 | GST expression vector | GenBank™ accession number U78872 |
| pPL2710 | GST-Hse1-SH3 | Ref. 20 |
| pPL2831 | GST-Pex13-SH3 | Ref. 20 |
| pPL3164 | GST-Hse1-SH3*. GST-Hse1-SH3 plasmid containing in which the codons encoding W254A and W255A mutations. | Ref. 20 |
| pPL1978 | GST-Vps27 C terminus | Ref. 3 |
| pPL833 JLU34 | STE3-GFP | Ref. 41 |
| pPL3607 | STE3-mCherry. Derived from pPL833. | This study |
| pGFP-Ub | GFP-Ub expressed from the PRC1 promoter in pRS426. | D. Katzmann Mayo Clinic (Rochester, MN) |
| pPL3601 | LEU2 marker convert of pGFP-Ub. | This study |
| pPL3155 | YEp351 carrying UBI4. | This study |
| pPL3327 | DOA1-GFP downstream of PRC1 promoter and upstream of the PHO8 3′-UT in pRS316. | This study |
| pPL2794 | GAL1 driving full-length DOA1 tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3270 | GAL1 driving truncated DOA1 (residues 1–300) tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3273 | GAL1 driving truncated DOA1 (residues 1–470) tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3359 | GAL1 driving truncated DOA1 (residues 1–414) tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3363 | GAL1 driving truncated DOA1 (residues 1–433) tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3341 | GAL1 driving truncated DOA1 (residues 1–445) tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3323 | GAL1 driving a fragment of DOA1 (residues 301–470) tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1. | This study |
| pPL3300 | GAL1 driving DOA1 tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1 Contains mutations p399PLKP → A399ALKA. | This study |
| pPL3367 | GAL1 driving DOA1 tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1 Contains mutations F434ILKNTN → A434AAKAAA to make doa1Δ1Hse1. | This study |
| pPL3371 | GAL1 driving DOA1 tagged C-terminally with V5 and His6 tag in 2μ plasmid pYES2.1 Contains deletion of residues 433–445 to make doa1Δ2Hse1. | This study |
| pPL2967 | Expression plasmid for PFU domain from wild type DOA1. Residues 301–466 with N-terminal V5 epitope tag in pET151. | This study |
| pPL3604 | Expression plasmid for PFU domain from Doa1Δ1Hse1. Fragment derived from pPL3367 (F434ILKNTN → A434AAKAAA) residues 301–466 with N-terminal V5 epitope tag in pET151. | This study |
| pPL3603 | Expression plasmid for PFU domain from Doa1Δ2Hse1. PFU domain from pPL3371 (deletion of residues 433–445) with N-terminal V5 epitope tag in pET151. | This study |
| pPL3498 | DOA1-V5 expressed from DOA1 promoter in pRS316. | This study |
| pPL3499 | Doa1Δ1Hse1 (F434ILKNTN → A434AAKAAA) expressed from DOA1 promoter in pRS316. | This study |
| pPL3501 | Doa1Δ2Hse1 (deletion of residues 433–445) expressed from DOA1 promoter in pRS316. | This study |
| pUG72 | Plasmid containing gene disruption cassettes with URA3 (K. lactis) as selection marker. | Ref. 40 |
| pUG27 | Plasmid containing gene disruption cassettes with his5+ (S. pombe) as selection marker. | Ref. 40 |
| pPL2161 | HA-tagged HSE1 in pRS315 | Ref. 3 |
| pPL1124 | VPS4 dominant negative in pRS426 | Ref. 20 |
| pGEX-6P-1 | GST expression vector | GenBank™ accession number U78872 |
| pPL1556 | VPH1-GFP-Ub in pRS316 | Ref. 41 |
| pYES2.1 | High copy (2μ) URA3-containing GAL1 expression plasmid. | Invitrogen |
| pET151 | T7 promoter-based bacterial expression vector. | Invitrogen |