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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Tetrahedron Lett. 2008;49(19):3045–3048. doi: 10.1016/j.tetlet.2008.03.063

Figure 3.

Figure 3

Fluorescence response of compound 1 (5 μM) to various reactive oxygen species (ROS). Data shown are for 1.5 μM of OCl−, 50 μM of O2−, 50 μM of t-BuO·, and 100 μM of all other ROS. Hydrogen peroxide (H2O2), tert-butyl hydroperoxide (TBHP), and hypochlorite (OCl−) were diluted from 30%, 70% and 5% aqueous solutions, respectively. Superoxide (O2−) was added as solid KO2. Hydroxyl radical (·OH) and tert-butoxy radical (t-BuO·) were generated by reaction of 1mM Fe2+ with 100 μM H2O2 or 100 μM TBHP, respectively. Spectra were acquired in 0.1M phosphate buffer, pH 7.4, and all data were obtained after incubation with the appropriate ROS ar room temperature. Emission intensity was collected at 454 nm (λex = 332 nm).