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Subcellular distribution of wild-type and L339,340A mutant receptors stably expressed in HEK293-cells. Cells expressing wild-type (WT) or L339,340A mutant β2-adrenergic receptors were incubated without (control) or with 10 μM (−)-isoproterenol (+iso) for 5 min, fixed, and permeabilized, and β2-adrenergic receptors were visualized with specific antibodies. (Bar = 7 μm.)
