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. 1986;64(6):889–896.

Defined Plasmodium falciparum antigens in malaria serology

M S Gabra, D Grossiord, L H Perrin, A Shaw, A Cheung, I A McGregor
PMCID: PMC2490983  PMID: 3549031

Abstract

The study evaluates three enzyme-linked immunosorbent assays (ELISA) of malaria antigens suitable for use in large-scale epidemiological studies. Results obtained using sera from 567 persons from the Gambia indicated that the micro-ELISA method using parasitized red blood cell extract did not reliably quantitate antimalarial antibodies, especially in young children. In contrast, two micro-ELISA methods that employed purified, defined antigens (a polypeptide of Mr = 41 000 present in rhoptries, and a 31-1 fusion polypeptide corresponding to a merozoite surface antigen) permitted the precise determination of antimalarial antibodies in both adults and children. Problems and advantages associated with the use of the Mr = 41 000 and 31-1 antigens for the determination of antimalarial antibodies are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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