Figure 7.

PH induces proteolytic cleavage of a constitutively expressed 70-kDa hepatic protein in iNOS-null mice. To investigate hepatocyte apoptotic activity, in vivo cleavage of U1–70kD was assayed by immunoblotting with monospecific human sera recognizing U1–70kD. A signature 40-kDa peptide indicates apoptotic cleavage of the intact protein. Results shown are representative of those found in four different mice/group at 24 or 36 hr after PH. In this blot, each lane contains a sample from a different mouse at 36 hr after PH.