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Proposed mechanism for RNA-peptide fusion formation on the ribosome. (A) The ribosome initiates protein synthesis on the mRNA and translocates toward the end of the template. (B) When the ribosome reaches the end of the RNA ORF, translation stalls at the RNA/DNA junction. The puromycin then enters the A site of the ribosome and accepts the nascent peptide. (C) Once formed the mRNA-peptide fusion is purified from the translation reaction by affinity chromatography.
