Figure 3.

Incorporation of [³⁵S]Met into the Met (43-P), short myc (LP77), and long myc (LP154) translation templates. (A) Denaturing PAGE analysis of templates isolated from translation reactions. The gel shows templates isolated by dT₂₅ affinity chromatography with no treatment (lanes 1–4), treatment with RNase A (lanes 5–8), and treatment with both RNase A and Proteinase K (lanes 9–12). The positions of unmodified 30-P, 43-P, LP77, and LP154 are indicated by arrows at the side of the gel. The schematic below indicates the proposed composition of the fragments in the RNase-treated lanes. (B) Sequential isolation of long myc linker fusion (30-P fusion) fragments by thiopropyl (TP) Sepharose and dT₂₅ affinity chromatography. Lane 2 is fusion product purified on TP Sepharose followed by dT₂₅, whereas lane 3 is material purified by dT₂₅ only.