Figure 4.
Analysis and quantitation of fusion formation by immunoprecipitation. (A) Denaturing urea PAGE analysis of the 32P-labeled linker-peptide fusions isolated by immunoprecipitation-RNase A-kinase treatment of the translation reactions. Reactions containing no template or only the RNA portion of the long myc template (RNA124) show no fusion formation. Reactions containing the long myc template contain bands corresponding to long myc linker fusions (30-P fusion) indicated by the arrow. The amount of input template is indicated at the top. (B) Recovered unmodified linker 30-P (left y axis) is linearly proportional to input template (x axis), whereas linker-peptide fusion (right y axis) is constant, as determined by PhosphorImager counting.