Table 5.

Effect of TRPM8 knockdown on cold (18°C)-induced cytokine levels

Gene	BEAS-2B Cells
	Time of Maximum Increase, h	Maximum Response, fold control ±SD	TRPM8shRNA + Cold (18°C)
IL-1α	4	3.8±0.1*	1.10±0.02#
IL-1β	0.17	4.4±0.4*	1.00±0.02#
IL-4	0.5	2.7±0.2*	0.60±0.02#
IL-6	4	16.5±1.3*	1.2±0.1#
IL-8	4	28±5*	1.9±0.2#
IL-10	4	3.9±0.6*	0.8±0.3#
IL-13	0.083	1.5±0.1	0.9±0.3
GM-CSF	2	3.1±0.3*	1.10±0.03#
TNF-α	4	12±1*	1.3±0.1#

^*Statistically significant increase relative to untreated control (paired t-test, P ≤ 0.05; n = 3);

^#statistically significant decrease relative to cold (paired t-test, P ≤ 0.05; n = 3). Treatments consisted of untreated controls and cold (18°C) exposure for various time points (0–4 h) with and without TRPM8 knockdown using TRPM8_shRNA targeted to exon 18.