Figure 4.

Total acute 0.3 M saline intake (± SEM) in rats sodium depleted 2 times compared to vehicle treated rats. Rats were sodium or sham depleted 3 times, each depletion spaced one week apart and given access to 0.3 M saline 24 h after the first 2 depletions. Total intakes were recorded over a 2 h period. Acute saline intake was significantly greater during the second sodium depletion as compared with the first in 3F rats (3F=3 furosemide treatments; 3V = 3 vehicle treatments). Rats were sacrificed for immunohistochemical processing of brain tissue after the third sodium depletion and were not given access to saline.*p < 0.05 as compared to first sodium appetite test.