Figure 5.
Photomicrographs of nucleus accumbens shell (first row), and subfornical organ (second row) depicting Fos-immunoreactivity (Fos-ir). Na+ depleted rats with a prior history of three Na+ depletions had increased levels of Fos-ir in the shell of the nucleus accumbens and in the subfornical organ compared to rats depleted once and vehicle-treated controls. A&E from rat treated with furosemide 3 times (3F); B&F from rat with two vehicle treatments + 1 furosemide treatment (2V1F); C&G from rat treated with vehicle 3 times (3V); D&H schematic drawings of areas counted [205], hatched bars define boundaries. First row: AcbC = nucleus accumbens, core; AcbSh = nucleus accumbens, shell; ICj = islands of Calleja; LAcbSh = nucleus accumbens, shell, lateral division; VP = ventral pallidum (bregma 1.20 mm). Second row: cc = corpus callosum; D3V = dorsal 3rd ventricle; Fi = fimbria of the hippocampus; LV = lateral ventricle; SFO = subfornical organ (bregma -1.40 mm). (Reprinted from [17] with permission.)