Figure 6.
(A) Mean (± SEM) Fos-immunoreactivity (Fos-ir) counts/mm2 for furosemide- and vehicle-treated rats with no access to saline and water. BLA=basolateral amygdala; CeA=central amygdala; mPFC=medial prefrontal cortex; NAc=nucleus accumbens Rats with multiple sodium depletions had significantly more Fos-ir in the BLA and NAc compared to rats depleted once and sham depleted rats. (B); PVN=paraventricular nucleus; SFO=subfornical organ; SON=supraoptic nucleus. 3V=3 vehicle treatments; 2V1F=2 vehicle treatments + 1 furosemide treatment; 3F=3 furosemide treatments. Rats depleted multiple times with furosemide had significantly more Fos-ir in the SFO than rats depleted once or sham depleted rats.*Significantly different as compared to 3V and as †compared to 2V1F. (Reprinted from [17] with permission.)