Table 3.

The growth and amount of phosphate removal per unit OD₆₆₀ of the yeast strains

Strain	OD660	Residual P (mg P/l)	Removal P per OD660 (mg P/l·OD660)
H. fabianii J640 (wild strain)	32.1	62.7	4.3
H. fabianii J640 PFW1	17.7	59.2	8.4
H. fabianii J640 PFW2	17.2	46.2	9.0
H. fabianii J640 PFW3	12.0	60.5	11.7
H. fabianii J640 PFW4	23.9	23.4	7.4
H. anomala J224-1 (wild strain)	24.3	69.4	4.8
H. anomala J224-1 PAW1	17.1	58.4	8.4

Each strain was incubated in 5 ml of YM medium with shaking for 48 h. The cells were harvested, inoculated into 50 ml of high P-YPAD medium (OD₆₆₀ = 0.2) in a 200-ml flask, and grown at 30°C with shaking at 120 rpm for 24 h. The growth was measured by OD₆₆₀, and residual phosphorus was measured in the cultivation media. Removal of phosphorus per OD₆₆₀ was calculated removal phosphorus, phosphorus concentration of initial media make allowance after cultivation media, divided by OD₆₆₀ ((initial P − residual P) / OD₆₆₀).